The following biomaterials can be ordered from the repository. Instructions for ordering can be found here. Statistics including the number subjects available with various cell types can be found here.

Cryopreserved Lymphocytes (CPLs)

Cryopreserved lymphocytes are isolated by gradient centrifugation of blood and aspiration of the peripheral blood mononuclear (PBMC) layer with subsequent cryopreservation in freezing media and using a computer-controlled rate freezer.

CPLs are ideal for creating induced pluripotent stem cells (iPSCs). While many somatic cell types such as skin fibroblasts are amenable to reprogramming to iPSCs, the best source cell type is cryopreserved lymphocytes. There are highly efficient protocols in place at Sampled for converting the erythroblast cell fraction of the CPL to iPSC. Erythroblasts have the advantage of not having genomic rearrangements as do T cells and also exhibit less genomic instability and mosaicism than fibroblasts because of the relative exposure of the skin to environmental factors.

Lymphoblastoid cell lines (LCLs)

Lymphoblastoid cell lines are derived by transformation of B lymphocytes using Epstein Barr Virus (EBV).

Whole Blood

Aliquots of whole blood are reserved on receipt and stored at -80°C.

Plasma

The plasma fraction of whole blood is isolated by centrifugation and aliquoted for storage at -80°C.

Genomic DNA (gDNA)

Genomic DNA (gDNA) is extracted from LCLs (see above).

Whole-Blood gDNA (WB gDNA)

This is gDNA extracted from whole blood (comprised of gDNA from all nucleated cells in the blood).